Salary and benefits are competitive. Salary is commensurate with qualifications and experience.
As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here.
The University of Washington (UW) is proud to be one of the nation's premier educational and research institutions. Our people are the most important asset in our pursuit of achieving excellence in education, research, and community service. Our staff not only enjoys outstanding benefits and professional growth opportunities, but also an environment noted for diversity, community involvement, intellectual excitement, artistic pursuits, and natural beauty.
The Department of Electrical Engineering at the University of Washington is a nationally ranked education and research program. Located on the main Seattle campus of the University of Washington, UW Electrical Engineering currently supports greater than 750 undergraduate and graduate students and over 50 active research faculty. EE's annual expenditures reach approximately $19 million and its external funding is close to $25 million. The Department has several key areas of research: communications/networking, electromagnetics/remote sensing, big data, integrated systems/circuits/VLSI, nanotech and photonics, power and energy, speech/image/video processing and systems/controls and robotics. Supporting over 470 undergraduate, 300 graduate and 100 professional students in their educational efforts, the Department has proven to be a leader in both education and research areas, as well as effectively leveraging intellectual and entrepreneurial engagement at all stages of research.
The Department of Electrical Engineering has an outstanding opportunity for a Research Scientist/Engineer 2 as a Biologist. The main purpose of this position is to perform single-cell RNA-sequencing (scRNA-seq) experiments using the SPLiT-seq protocol and to work with graduate students and postdocs in the lab to optimize that protocol. SPLiT-seq is an affordable, instrument-free approach for scaling up scRNA-seq experiments to very large cell numbers that was recently developed in the Seelig lab. SPLiT-seq currently is a multi-day workflow that requires experience with molecular biology and biochemistry techniques. More information about the technology and protocol can be found here: https://sites.google.com/uw.edu/splitseq.
Perform SPLiT-seq workflow (cell culture, cell fixation and permeabilization, reverse transcription of RNA in fixed cells, combinatorial barcoding of the cDNA, sequencing library prep).
Work directly with Principal Investigator, postdoctoral fellows and graduate students to perform and optimize experiments.
Assist in the design of scientific experiments; provide background data and information to determine the correct and accurate scientific methodology and experimental steps. Clearly organize and document research steps and methods.
Assist lab staff with review and analysis of scientific results.
Responsible for the management and inventory of laboratory supplies used for the SPLiT-seq experiments. Coordinate purchasing of all supplies utilized by the SPLiT-seq team.
Prepare SPLiT-seq reagent stocks.
Gain experience in performing single-cell RNA-seq experiments.
Opportunity to learn and employ data analysis skills.
Become a co-author of scientific publications.
Bachelor's degree or higher in molecular biology, biochemistry or a related field.
At least two years of experience working in a molecular biology or biochemistry laboratory.
Previous experience with mammalian cell culture
Previous experience working with RNA derived from cell lines or similar samples (RNA purification, RT-qPCR,)
Strong organizational and communication skills.
Ability to critically evaluate experimental workflows.
Ability to collect data in a consistent, reliable and accurate manner.
Demonstrated ability to work as part of a collaborative team.
Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.
Previous experience with next generation RNA-seq experiments using the Illumina NextSeq or MiSeq platform.
Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Criminal Conviction History, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select Apply to this position. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your My Jobs page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
Founded in 1861, the University of Washington is one of the oldest public institutions in the west coast and one of the preeminent research universities in the world. The University of Washington is a multi-campus university comprised of three different campuses: Seattle, Tacoma, and Bothell. The Seattle campus is made up of sixteen schools and colleges that serve students ranging from an undergra...duate level to a doctoral level. The university is home to world-class libraries, arts, music, drama, and sports, as well as the highest quality medical care in Washington State and a world-class academic medical center. The teaching and research of the University’s many professional schools provide undergraduate and graduate students the education necessary toward achieving an excellence that will serve the state, the region, and the nation. As part of a large and diverse community, the University of Washington serves more students than any other institution in the Northwest.